In this manuscript, we aimed to report the synthesis of aluminum (Al) incorporated carbon nanodots (CD) and their activities on the immune cells. A green synthesis method involving the in-situ doping of the nanodot was conducted. Synthesized nanodots immunomodulatory and immunostimulatory activities were tested invitro on the macrophages. The produced
carbon dots were water-soluble, fluorescent and monodispersed, with an average diameter of around 10–20nm. After Al-doping, their surface properties, stability, crystallinity, as well as their fluorescent and optical properties were evaluated.
These Al-CDs displayed no cytotoxicity and enhanced the pro-inflammatory activities of the mammalian macrophages with much lower aluminum concentrations (‰ 20) compared to that of conventional aluminum salt, by virtue of which they have the potential to serve as the safe and effective adjuvant carrier. The stability of the nanocarriers was found to be persistent for over 3 months at room temperature with no significant formation of the aggregates. These results support the promise of such nanodots as the new generation non-toxic adjuvant candidates
Cite This Article: Ayaz, F et al. (2019) Molecular Biology Reports, https://doi.org/10.1007/s11033-019-04701-1
Carbon dots are often synthesized in the presence of a carbon source and passivating agents in which they are crucial for an enhanced fluorescence. The solvent choice and/or combination to be used in the synthesis of these nanoparticles can influence their surface chemical composition, morphology, and fluorescence properties. In this study, highly fluorescent carbon dots were synthesized using deep eutectic solvents of different compositions as green solvent media and doping agent. Resulting carbon dots were then separated by their hydrophilicity/hydrophobicity using a three-phase solvent system (water/acetone/chloroform) and compared with traditional centrifugation-based separation method. Carbon dots with a size below 20 nm and quantum yield reaching 50% were obtained. Many properties of them including surface functional groups, optical, fluorescence, and electric properties were shown to be determined by the deep eutectic solvent composition.
Journal of Materials Science 53(4) (2018) DOI: 10.1007/s10853-018-2723-4